S.L. 437.63 Art. 1Title, scope and applicability. 1. 1. This molecular checking Directive 2. The method hot with rotation of the In the filtrate with measured as The gives the 3. 3.1. 25% 3.2 1. 28% The 0,1 mol/litre 30.94 ml of 3.3 and 3 g of 3.4 (Fe(CN) ].3H 6 3.5 40% 4. 4.1. 250 condenser. 4.2. 5. 5.1. Crush the r

(1) The title of these rules is the Methods for Analysis in the Official Control of Feedingstuffs Rules.

(2) The scope for these rules is to implement the rules found under EU Commission Directive 72/199/EEC establishing methods of analysis for the official control of feedingstuffs.

(3) Malta shall require that analyses for official controls of feedingstuffs, as regards their level of starch, crude protein, crude protein which can be dissolved by Pepsin and hydrochloric acid, of free and total gossypol and as regards pepsin activity be carried out using the methods described in Schedule I.

(4) Malta shall require that analyses for official controls of feedingstuffs for the purpose for determining the levels of tylosin, and virginiamycin in feedingstuffs, shall be carried out using the methods described in Schedule II.

*These rules have been repealed by Legal Notice 37 of 2025. SCHEDULE I

DETERMINATION OF STARCH POLARIMETRIC METHOD Purpose and scope method makes it possible to determine the levels of starch and of high weight starch degradation products in feedingstuffs for the purpose of compliance with EU Commission Directive 86/174/EEC and EU Council 96/25/EC. Principle comprises two determinations. In the first, the sample is treated when dilute hydrochloric acid. After clarification and filtration the optical solution is measured by polarimetry. second, the sample is extracted with 40% ethanol. After acidifying the hydrochloric acid, clarifying and filtering, the optical rotation is in the first determination.

difference between the two measurements, multiplied by a known factor, starch content of the sample.

Reagents (w/w) hydrochloric acid, d: 1,126 g/ml. (w/v) hydrochloric acid.

concentration must be checked by titration using a sodium hydroxide solution in the presence of 0,1% (w/v) methyl red in 94% (v/v) ethanol. 10 ml = NaOH 0.1 mol/lite. Carrez solution I: dissolve 21,9 g of zinc acetate Zn (CH COO) 2H O 3 2 2 glacial acetic acid in water. Make up to 100 ml with water. Carrez solution II: dissolve 10,6 g of potassium ferrocyanide [K 4 O in water. Make up to 100 ml with water. 2 (v/v) ethanol, d: 0,948 g/ml at 20ºC.

Apparatus ml erlenmeyer flask with standard ground-glass joint and with reflux

Polarimeter or saccharimeter. Procedure

Preparation of the sample sample until it is fine enough for all of it to pass through a 0,5 mm sieve. Determination of the total optical rotation (P or S) (see observation 7.1) g of the crushed sample to the nearest mg and place in a 100 ml flask. Add 25 ml of hydrochloric acid (3.2), shake to obtain even of the test sample and add a further 25 ml of hydrochloric acid (3.2). flask in a boiling water bath shaking vigorously and steadily for the minutes to prevent the formation of agglomerates. The quantity of water in must be sufficient for the bath to remain at boiling point when the flask is introduced into it. The flask must not be taken out of the bath shaken. After exactly 15 minutes, remove from the bath, add 30 ml of cool immediately to 20ºC. Add 5 ml of Carrez solution I (3.3) and shake for one minute. Then Carrez solution II (3.4) and shake again for one minute. Make up to water, mix and filter. If the filtrate is not perfectly clear (which is rare), determination using a larger quantity of Carrez solutions I and II, for Measure the optical rotation of the solution in a 200 mm tube with or saccharimeter. 5.3. Determination of the optical rotation (P' or S') of substances 40% ethanol Weigh 5 g of the sample to the nearest mg, place in a 100 ml graduated add about 80 ml of ethanol (3.5) (see observation 7.2). Leave the flask hour at room temperature; during this time, shake vigorously on six occasions the test sample is thoroughly mixed with the ethanol. Make up to ethanol (3.5), mix and filter. Pipette 50 ml of the filtrate (= 2,5 g of the a 250 ml erlenmeyer flask, add 2,1 ml of hydrochloric acid (3.1) vigorously. Fit a reflux condenser to the erlenmeyer flask and immerse boiling water bath. After exactly 15 minutes, remove the erlenmeyer bath, transfer the contents to a 100 ml graduated flask, rinsing with water, and cool to 20ºC. Clarify using Carrez solutions I (3.3) and II to volume with water, mix, filter and measure the optical rotation as indicated second and third paragraphs of 5.2.