S.L. 437.77 Art. 4or equivalent, length: 300 (the quantities (3.15) into a Spread the Leave to dry time of use, obtained with must on

Apparatus 4.1. Grinder-mixer.

4.2. Shaking apparatus or magnetic stirrer. 4.3. Fluted filter papers, Schleicher and Schüll No 588 diameter: 24 cm 4.4. Glass tube for chromatography (internal diameter: 22 mm, mm), with a PTFE cock and a 250-ml reservoir. 4.5. Rotary vacuum evaporator, with a 500-ml round-bottom flask. 4.6. 500-ml conical flasks, with ground-glass stoppers.

4.7. TLC apparatus. 4.8. Glass plates for TLC, 200 × 200 mm, prepared as follows indicated are sufficient to cover five plates). Put 30 g of silica gel G-HR conical flask. Add 60 ml water, stopper and shake for a minute. suspension on the plates so as to obtain a uniform layer 0ꞏ25 mm thick. in the air and then store in a desiccator containing silica gel. At the activate the plates by keeping them in the oven at 110ºC for one hour.

Ready-to-use plates are suitable if they give results similar to those the plates prepared as indicated above.

4.9. Long-wavelength (360 nm) UV lamp. The intensity of irradiation make it possible for a spot of 1 ng of aflatoxin B to be still clearly distinguished 1 a TLC plate at a distance of 10 cm from the lamp. 4.10. 10 ml graduated tubes with polyethylene stoppers.

4.11. UV spectrophotometer. 4.12. Fluorodensitometer (optional).