S.L. 437.77 · Methods Of Analysis For The Official (S.L. 437.77)
S.L. 437.77 Art. 6at 5.4.2.2. the separating 1 g 4 cm) can approximately collect the dryness in the of chloroform. 5.6.2.
Confirmatory test 6.1. Further treatment of the extract (5.4.2.2) Add 5 ml sodium chloride solution (3.15.1) to the final extract obtained Extract three times each with 2 ml chloroform (3.1) for one minute, in funnel (4.24.1). Pour the combined chloroform extracts over approximately sodium sulphate (3.15.2) into a 10 ml test tube. A small funnel (diameter: be used with a piece of cottonwool in the constriction, covered with a 1 g sodium sulphate. Wash the sodium sulphate layer with a few ml of chloroform and washing in the same test tube. Evaporate the chloroform extract to same test-tube using the heating block (4.24.2) and redissolve in 1 ml
6.2. Preparation of derivative and thin layer chromatography: See Annex to European Council Directive 76/372/EEC method A, point