S.L. 437.55 ст. 3Role of laboratories. Conditions for 1. These (defined as fish) in framework of in accordance Schedule are variant or animal Furthermore, constituents analyses are programme. 2. Dependent (< 0,1%) in 3. A Commission methods of undergone is fit for higher than materials (e.g. animal origin characteri

(1) Laboratories carrying out official controls on the presence of animal constituents in feedingstuffs shall participated periodically in proficiency testing on the analytical methods.

(2) Laboratory personnel carrying out analyses shall receive adequate training.

*These rules have been repealed by Legal Notice 37 of 2025. FOR THE OFFICIAL CONTROL OF FEEDINGSTUFFS

SCHEDULE the microscopic detection, identification or estimation of constituents of animal origin in feedingstuffs Objective and field of application conditions shall be used when detection of constituents of animal origin products from processing bodies and body parts of mammals, poultry and feedingstuffs is carried out by means of microscopic examination in the the coordinated inspection programme in the field of animal nutrition with Council Directive 95/53/EC. Provided that the methods in this used in all official tests, a second test may also be carried out using alternative methods, in order to improve the detection of certain types of constituents or to specify further the origin of the animal constituents. a variant protocol may be used when examining certain specific animal such as plasma or bones in tallow (see also point 9), provided that these made in addition to the analyses foreseen in the coordinated inspection

Sensitivity on the nature of the constituents of animal origin, very small amounts feedingstuffs can be detected. Principle representative sample, taken in accordance with the provisions laid down in Directive 76/371/EEC of 1 March 1976 establishing Community sampling for the official control of feedingstuffs (1) which has suitable preparation is used for the identification. The following protocol handling feed with low moisture content. Feed with an amount of moisture 14% shall be dried (condensed) prior to handling. Special feed or feed fats, oils) need dedicated treatment (see point 9). The constituents of are identified on the basis of typical, microscopically identifiable (i.e. muscle fibres and other meat particles, cartilage, bones, horn, blood, feathers, egg shells, fish bones, scales). The identification has to on the sieve fraction (6.1) and the concentrated sediment (6.2) of the

Reagents

Embedding agent Chloral hydrate (aqueous, 60% w/v)

(NaOH 2,5% w/v or KOH 2,5% w/v) for sieve fractions Paraffin oil or glycerol (viscosity: 68-81) for microscopic observations in agents Alcohol, 96%

Acetone

Concentrating agent Tetrachloroethylene (density 1,62)

Staining reagents FOR THE OFFICIAL CONTROL OF FEEDINGSTUFFS [

4.4.1. Iodine/potassium iodide solution (Dissolve 2 g potassium iodide water and add 1 g iodine while shaking frequently) 4.4.2. Alizarin Red (Dilute 2,5 ml 1M hydrocloric acid in 100 ml 200 mg alizarine red to this solution) 4.4.3. Cystine reagent (2 g lead acetate, 10 g NaOH/100 ml H2O) 4.4.4. Iodine/potassium iodide solution (dissolved in 70% ethanol)

4.5. Bleaching reagent 4.5.1. Commercial sodium hypochlorite solution (9.6% active chlorine)