S.L. 437.55 ст. 7point are used amount (and contain bone and birds) can slide by origin in the concentrated origin. at least five as a rule but also other fragments and portion of the estimated of the sample percentage of case of meat scale contents the order of 10 to estimate = (S × c)/(W × × f) × 100 portion of (%

Calculation and evaluation Member States shall ensure that the procedures described in this where an official analysis is carried out with a view to estimating the not simply the presence) of animal constituents. The calculation can only be made if the constituents of animal origin fragments. Bone fragments of terrestrial warm-blooded species (i.e. mammals be distinguished from the different types of fish bone on the microscopic means of the typical lacunae. The proportion of constituents of animal sample material is estimated taking into consideration: - the estimated proportion (weight %) of bone fragments in the sediment and - the proportion (weight %) of bone in the constituents of animal

The estimate has to be based on at least three (if possible) slides and fields per slide. In compound feedingstuffs, the concentrated sediment contains not only terrestrial animal bone and fish bone fragments, particles of high specific weight, e.g. minerals, sand, lignified plant the like.

7.1. Estimated value of the percentage of bone fragments % terrestrial bone fragments = (S × c)/W % fish bone and scale fragments = (S × d)/W (S = sediment weight (mg), c = correction factor (%) for the estimated terrestrial animal bones in the sediment, d = correction factor (%) for portion of fish bones and scale fragments in the sediment, W = weight material for the sedimentation (mg)).

7.2. Estimated value of constituents of animal origin The proportion of bone in animal products can vary greatly. (The bone in the case of bonemeals is of the order of 50 to 60% and in the meals of the order of 20 to 30%; in the case of fish meals bone and vary according to the category and origin of the fishmeal, normally in to 20%). If the type of animal meal present in the sample is known, it is possible the content: Estimated content of constituents of terrestrial animal products (%) f) × 100 Estimated content of constituents of fish products (%) = (S × d)/(W (S = sediment weight (mg), c = correction factor (%) for the estimated terrestrial animal bone constituents in the sediment, d = correction factor FOR THE OFFICIAL CONTROL OF FEEDINGSTUFFS

portion of fish bones and scale fragments in the sediment, f = correction proportion of bone in the constituents of animal origin in the sample = weight of the sample material for the sedimentation (mg)).

Expression of the result of the examination shall at least contain information on the presence of constituents terrestrial animals and from fishmeal. The different cases shall be following way:

regard to the presence of constituents derived from terrestrial animals: as was discernible using a microscope, no constituents derived from animals were found in the submitted sample; or as was discernible using a microscope, constituents derived from animals were found in the submitted sample.

regard to the presence of fishmeal: as was discernible using a microscope, no constituents derived from in the submitted sample; or as was discernible using a microscope, constituents derived from fish the submitted sample.

constituents derived from fish or terrestrial animals are found, the report of result, if required, can further indicate an estimation of the amount constituents detected (x %, < 0,1%, 0,1-0,5%, 0,5-5% or > 5%), further of the type of terrestrial animal if possible and the animal constituents (muscle fibres, cartilage, bones, horn, hair, bristles, feathers, blood, egg bones, scales). where the amount of animal ingredients is estimated the correction shall be mentioned. where bone constituents from terrestrial animals are identified, the contain the additional clause: possibility that the above constituents are derived from mammals cannot be additional clause is not necessary in cases where the bone fragments from animals have been specified as bone fragments from poultry or mammals. Optional protocol for analysing fat or oil following protocol may be used for the analysis of fat or oil:

fat is solid, it is warmed for example in a microwave oven until it is a pipette, 40 ml of fat is transferred from the bottom of the sample to tube.

Centrifuge for 10 minutes at 4 000 rpm. fat is solid after centrifugation, it is warmed once more in an oven liquid. Repeat the centrifugation for five minutes at 4 000 rpm. a small spoon or a spatula, one half of the decanted impurities is a small petri dish or a microscopic slide for microscopic identification content of animal constituents (meat fibres, feathers, bone fragments). embedding agent for microscopy, paraffin oil or glycerol is recommended. FOR THE OFFICIAL CONTROL OF FEEDINGSTUFFS [

- The remaining impurities are used for sedimentation as described 6.2.